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cell id intercalator ir  (fluidigm)


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    Structured Review

    fluidigm cell id intercalator ir
    Cell Id Intercalator Ir, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+id+intercalator+ir/Cell-ID+Intercalator-Ir/pmc13044534-113-9-13
    Average 96 stars, based on 216 article reviews
    cell id intercalator ir - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Peripheral Treg-monocyte immune signatures relate to neurodegeneration and prognosis in patients with primary tauopathies
    Article Snippet: .. After discarding the supernatant, the samples were resuspended in 1 mL of 125nM Cell-ID Intercalator-Ir (Cat# 201240, Standard BioTools) in Maxpar Fix & Perm buffer (Cat#: S00092, Standard BioTools) and left for staining overnight in the fridge at 4°C. ..

    Article Title: High-Dimensional Protein Analysis Uncovers Distinct Immunologic and Stromal Features in Primary and Metastatic Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. Following intracellular staining, cells were washed and incubated with Cell-ID Intercalator-Ir 125 μm (Fluidigm Sciences, #201192B). .. Samples were acquired on a Helios mass cytometer (Fluidigm Sciences), and raw flow cytometry standard (FCS) files were normalized using the CyTOF software normalizer algorithm.

    Article Title: MIMIC: a flexible pipeline to register and summarize IMC-MSI experiments.
    Article Snippet: .. After staining, cells were washed twice with phosphate-buffered saline (PBS) and then simultaneously fixed while staining DNA using Cell-ID Intercalator Ir (Standard BioTools PN: 201192B) diluted 1:1000 in Maxpar Fix and Perm Buffer (Standard BioTools PN: 201067) for thirty minutes. ..

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: .. After staining, cells were washed twice with CSM, and then stained with Cell-ID Intercalator-Ir (Fluidigm Corporation; 191Ir and 193Ir) at a final concentration of 125 nM in 1000 μL of 1.5% fresh paraformaldehyde (diluted in 1x PBS) overnight at 4 °C for DNA staining. .. Finally, cells were resuspended in Milli-Q water containing EQTM Four Element Calibration Beads (Fluidigm Corporation, San Francisco, CA, USA) and acquired on a CyTOF2 mass cytometer (Fluidigm Corporation, San Francisco, CA, USA).

    Incubation:

    Article Title: High-Dimensional Protein Analysis Uncovers Distinct Immunologic and Stromal Features in Primary and Metastatic Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. Following intracellular staining, cells were washed and incubated with Cell-ID Intercalator-Ir 125 μm (Fluidigm Sciences, #201192B). .. Samples were acquired on a Helios mass cytometer (Fluidigm Sciences), and raw flow cytometry standard (FCS) files were normalized using the CyTOF software normalizer algorithm.

    Article Title: Immune-microbiome coordination defines interferon setpoints in healthy humans.
    Article Snippet: Intracellular staining was then performed using eBioscience Permeabilization Buffer (Invitrogen) at 4◦C for 60 minutes on a shaker set on 90 RPM. .. Cells were later incubated with Cell-ID Intercalator-Ir (Standard Biotools) at 0.0625 uM and 4% PFA overnight at 4◦C. .. Before running, cells were resuspended in cell acquisition solution (Standard Biotools) that contained EQ Four Element Calibration Beads (Standard Biotools).

    Saline:

    Article Title: MIMIC: a flexible pipeline to register and summarize IMC-MSI experiments.
    Article Snippet: .. After staining, cells were washed twice with phosphate-buffered saline (PBS) and then simultaneously fixed while staining DNA using Cell-ID Intercalator Ir (Standard BioTools PN: 201192B) diluted 1:1000 in Maxpar Fix and Perm Buffer (Standard BioTools PN: 201067) for thirty minutes. ..

    Concentration Assay:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: .. After staining, cells were washed twice with CSM, and then stained with Cell-ID Intercalator-Ir (Fluidigm Corporation; 191Ir and 193Ir) at a final concentration of 125 nM in 1000 μL of 1.5% fresh paraformaldehyde (diluted in 1x PBS) overnight at 4 °C for DNA staining. .. Finally, cells were resuspended in Milli-Q water containing EQTM Four Element Calibration Beads (Fluidigm Corporation, San Francisco, CA, USA) and acquired on a CyTOF2 mass cytometer (Fluidigm Corporation, San Francisco, CA, USA).

    other:

    Article Title: Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization.
    Article Snippet: Prior to the acquisition, cells were washed twice with CSB and twice with MaxPar Cell Acquisition Solution Plus (CAS PLUS, 201244, Standard Biotools) at 800 x g for 5 min at 4◦C, then resuspended at 6 × 105 per mL in CAS PLUS.



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